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Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR

机译：多重PCR同时检测三种主要鱼类病原体沙门氏菌，精神黄杆菌和rersinia ruckeri

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A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.

机译：开发了一种基于16S rRNA基因的多重PCR分析方法，用于同时检测三种主要鱼类病原体，即鲑鱼气单胞菌，精神黄杆菌和ruckeria ruckeri。该检测方法被证明具有特异性，并且与每种单次PCR检测方法一样灵敏，分别对鲑鱼曲霉，嗜热链球菌和R. ruckeri的检出限分别为6、0.6和27 CFU。该测定法可用于检测人工感染鱼以及养殖场暴发中的细菌。结果表明，该多重PCR系统为常规的实验室诊断由这三种细菌产生的感染提供了一种特异性，灵敏，可重现和快速的方法。

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Cerro, A.; Márquez, I.; Guijarro, J.A. (J.);
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年度 2012
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1. Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR [J] . A. del Cerro, I. Marquez, J. A. Guijarro Applied and Environmental Microbiology . 2002,第10期

机译：多重PCR同时检测三种主要鱼类病原体沙门氏菌，精神黄杆菌和rersinia ruckeri
2. Development and evaluation of a multiplex PCR assay for simultaneous detection of Flavobacterium psychrophilum, Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida in culture fisheries [J] . Ertan Emek Onuk, Alper Ciftci, Arzu Findik, Journal of Veterinary Science . 2010,第3期

机译：同时检测精神黄杆菌，ruckeria ruckeri和沙门氏菌亚种的多重PCR分析方法的开发和评估。养殖渔业中的鲑科
3. Optimization of nested polymerase chain reaction assays for identification of Aeromonas salmonicida, Yersinia ruckeri, and Flavobacterium psychrophilum [J] . Taylor PW, Winton JR Journal of Aquatic Animal Health . 2002,第3期

机译：嵌套式聚合酶链反应测定法的优化，可用于鉴定鲑鱼气单胞菌，ruckeria ruckeri和嗜冷黄杆菌
4. DETECTION OF FIVE PATHOGENS IN PORCINE LUNG SAMPLES BY THE NEWLY DEVELOPED MULTIPLEX LIGATION DEPENDENT PROBE AMPLIFICATION TECHNOLOGY (MLPA MULTIPLEX PCR) [C] . GJ. Wellenberg, M. Reijans, C. Schouten, Congress of the International Pig Veterinary Society . 2008

机译：通过新开发的多重连接依赖性探针扩增技术检测猪肺样品中五种病原体（MLPA多重PCR）
5. Detection of fish pathogens with PCR-DGGE in non-lethal mucus samples and molecular typing of Aeromonas salmonicida using the 16S-23S internal transcribed spacers. [D] . Quinn, Robert Andrew. 2008

机译：利用PCR-DGGE检测非致死性粘液样本中的鱼类病原体，并使用16S-23S内部转录间隔子对鲑鱼气单胞菌进行分子分型。
6. Simultaneous Detection of Aeromonas salmonicida Flavobacterium psychrophilum and Yersinia ruckeri Three Major Fish Pathogens by Multiplex PCR [O] . A. del Cerro, I. Marquez, J. A. Guijarro 2002

机译：多重PCR同时检测三种主要鱼类病原体沙门氏菌精神黄杆菌和rersinia ruckeri
7. Simultaneous detection of Aeromonas salmonicida;Flavobacterium psychrophilum;and Yersinia ruckeri;three major fish pathogens;by multiplex PCR [O] . Cerro Arrieta, Ana del, Márquez Llano-Ponte, Isabel, Guijarro Atienza, José Agustín 2002

机译：多重PCR同时检测三种主要鱼类病原体：沙门氏菌气旋杆菌，嗜冷黄杆菌和ruckeria ruckeri

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Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR

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